Journal: Cell Death Discovery
Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke
doi: 10.1038/s41420-021-00773-w
Figure Lengend Snippet: A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of RBFox-1 in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.
Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).
Techniques: Immunostaining, Lactate Dehydrogenase Assay, Western Blot, Luciferase, Activity Assay, Transfection